Urea addition as an enhanced strategy for degradation of petroleum contaminants during co-composting of straw and pig manure: Evidences from microbial community and enzyme activity evaluation.

Alterations in microbial community succession patterns and enzyme activities by petroleum pollutants during co-composting of straw and swine manure with the supplementary nitrogen source are unclear. In this study, urea was added into co-composting systems, and the removal performance of petroleum, microbial enzyme activity and community changes were investigated. Results showed that the polyphenol oxidase and catalase activities which were both related to the degradation of petroleum contaminants were accordingly increased from 20.65 to 30.31 U/g and from 171.87 to 231.86 U/g due to urea addition. The removal efficiency of petroleum contaminants in composting with urea increased from 45.06% to 82.29%. The addition of urea increased the diversity and abundance of petroleum-degrading microorganisms, and enhanced microbial linkages. This study provides a novel strategy for the degradation of petroleum hydrocarbon as well as a new insight into the effect of urea on both microbial processes and composting phases.

Microalgae Chlorella vulgaris and Scenedesmus dimorphus co-cultivation with landfill leachate for pollutant removal and lipid production.

In this study, landfill leachate was pre-treated with NaClO, and then diluted to 5%, 10% and 15% for microalgae growth of Chlorella vulgaris and Scenedesmus dimorphus in the mono- and co-culture modes to investigate the nutrient removal and growth characteristics of microalgae. The results revealed that landfill leachate with the 10% dilution rate was conducive for microalgae growth and exhibited robust biomass growth and the highest nutrient removal efficiency. The co-culture biomass in 10% landfill leachate achieved 0.266 g/L within 10 days and demonstrated the improved nutrient utilisation efficiency of microalgae. In addition, the chemical oxygen demand, ammonia nitrogen, total nitrate and total phosphorus removal efficiencies accordingly reached 81.0%, 80.1%, 72.1% and 86.0% in 10% landfill leachate. Meanwhile, both the enzyme activity and fluorescence parameters proved that the cell activity of co-culture was higher than that of mono-culture.